Isolation of acidic lipoproteins from brain chromatin Their relation to the acidic nonhistone proteins
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چکیده
منابع مشابه
The isolation, properties, and possible functions of chromatin acidic proteins.
The chromatin acidic proteins have been isolated from rat liver by dissociation of the deoxyribonucleoprotein complex. These proteins are freely soluble in buffer solutions, are heterogeneous, contain deoxyribonucleic and ribonucleic acids, and are separable into four main fractions. The isolated chromatin acidic proteins form complexes with whole histone as well as with DNA. When they are incu...
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The multiple classes ofbinding sites for the progesterone-receptor complex in hen oviduct nuclei were found to be of chromatin origin. The highest-affinity, and presumably most physiologically important class, is localized in oviduct chromatin and contains approx. 6000-10000 sites per nucleus. None of these sites is detected in spleen chromatin. Two new techniques were used for assaying rapidly...
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In aqueous extracts of rat brain, spinal cord, and sciatic nerve, a protein antigen has been detected which could not be shown in similarly prepared extracts of a variety of other rat tissues. The protein, designated antigen (Y, had a high mobility in zone electrophoresis at pH 7.4. Antigen cr was isolated and was found to consist of a single type of subunit having a molecular weight of about 3...
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The acidic chromatin proteins extracted from normal and neoplastic mammary cells of C3H mice and Fischer rats have been compared by polyacrylamide gel electrophoresis, amino acid analysis, and radioactivity labeling patterns of synthesis in vitro. Following purification of chromatin from purified nuclei and extraction of histones in 2 M sodium chloride, the acidic proteins associated with DNA w...
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Mammalian bones are composed of calcium phosphate crystals in a protein matrix. The major form of the calcium phosphate is hydroxyapatite. The most abundant matrix protein in bone is type I collagen. Collagen contributes to the mechanical properties of bone and is necessary for calcification of the tissue. In addition to collagen, several acidic proteins are present as minor components. Osteoca...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1973
ISSN: 0014-5793
DOI: 10.1016/0014-5793(73)80700-8